Autoradiographic localisation of VIP receptors in human lung

FEBS Letters

Leys, K.¹  Sever, P.S.¹  Madonna, O.¹  Morice, A.H.¹ 

[1] Department of Clinical Pharmacology, St. Mary's Hospital Medical School, Norfolk Place, London W2, England

关键词: Hormone receptor;    (Human lung);    Autoradiography;    Vasoactive intestinal peptide;   

DOI  :  10.1016/0014-5793(86)80479-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Localisation and pharmacological properties of the VIP receptor in human lung sections are described. The receptor density determined by saturation analysis using ¹²⁵I-VIP is approx. 100 protein, with a k _d of approx. 600 pM. Inhibition of ¹²⁵I-VIP binding with VIP and related peptides gives a rank order of potency: VIP /s> peptide histidine methionine /s> secretin. Light microscope autoradiography reveals specific VIP binding sites, with a high density over the pulmonary artery smooth muscle and the alveolar walls and with a lower density over the bronchial epithelium.

【 授权许可】

Unknown