Rate constants for reaction of hydroxyl radicals with Tris, Tricine and Hepes buffers

FEBS Letters

Gebicki, Janusz M.¹  Hicks, Mark¹ 

[1] School of Biological Sciences, Macquarie University, North Ryde, 2113 NSW, Australia

关键词: Hydroxyl radical;    Organic buffer;    Hydroxyl radical scavenger;    Rate constant;    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethanesulphonic acid;    Tricine;    N-[2-hydroxy-1;    1-bis-(hydroxymethyl)ethyl]glycine;    Tris;    2-amino-2-hydroxy-methylpropane-1;    3-diol;   

DOI  :  10.1016/0014-5793(86)81230-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rate constants for the reactions of three commonly used organic buffers and hydroxyl radicals were measured using steady-state competition kinetics with thymine. For Hepes (4-(2-hydroxyethyl)-1-piperazine-ethanesulphonic acid), Tricine (N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine and Tris (2-amino-2-hydroxymethylpropane-1,3-diol) the rate constants were 5.1×10⁹, 1.6×10⁹ and 1.1 × 10⁹ 1·mol⁻¹·s⁻¹, respectively.

【 授权许可】

Unknown