The primary structure of ornithine aminotransferase

FEBS Letters

Barra, Donatella²  Bossa, Francesco²  Simmaco, Maurizio²  John, Robert A.¹ 

[1] Department of Biochemistry, University College, PO Box 78, Cardiff CF1 1XL, Wales UK;Istituto di Chimica Biologica e Centro di Biologia Molecolare del CNR, Universitá di Roma, 00185 Roma, Italy

关键词: Ornithine aminotransferase;    Active-site lysine;    Leader sequence;    Primary structure;   

DOI  :  10.1016/0014-5793(86)81219-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tentative assignments of functional residues in rat liver mitochondrial ornithine aminotransferase have recently been made using the amino acid sequence deduced from a cDNA clone [(1985) J.Biol.Chem. 260, 12993-12997]. Partial sequences obtained using the pure mature protein demonstrate that one of these assignments, that of Lys 292 as the residue that binds the coenzyme pyridoxal phosphate, is correct. However, the identification of the Glu 34-Gln 35 bond as the site of post-translational proteolysis is in error. This cleavage occurs instead at Ala 25-Thr 26.

【 授权许可】

Unknown