The secondary structures of the Xenopus laevis and human mitochondrial small ribosomal subunit RNA are similar

FEBS Letters

Brun, G.¹  Dunon-Bluteau, D.² 

[1] Laboratoire de Biochimie des acides nucléiques. Ecole Normale Supérieure de Saint-Cloud, 92211 Saint-Cloud Cedex, France;Laboratoire de Biochimie, Section de Biologie, Institut Curie, 26 rue d'Ulm, 75231 Paris Cedex 05 France

关键词: Mitochondria;    rRNA;    Secondary structure;    D-loop;    displacement loop;    kb;    1000 base pairs;    mt;    mitochondrial;    nt;    nucleotide;    PAGE;    polyacrylamide gel electrophoresis;    mt 12 S rRNA;    small ribosomal subunit RNA of vertebrate mitochondria;    16 S rRNA;    small ribosomal subunit RNA of Escherichia coli;    18 S rRNA;    cytoplasmic small ribosomal subunit RNA;   

DOI  :  10.1016/0014-5793(86)80431-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extensive corrections of the nucleotide sequence of the Xenopus laevis mitochondrial small ribosomal subunit RNA gene [Roe et al. (1985) J. Biol. Chem. 260, 9759-9774] are reported. We found an additional fragment of 142 nucleotides and describe 25 nucleotide differences scattered in the gene. The nucleotide sequence of the X. laevis mitochondrial 12 S rRNA gene presents 80% homology with that of the same gene of bovine mitochondrion. We propose a new secondary structure for the product of the X. laevis gene. Contrary to the finding of Roe et al., we observed the same general organization of stems and loops as for the human mitochondrial 12 S rRNA gene product. On the other hand, the structural homology observed between the mitochondrial and cytoplasmic small subunit rRNAs of X. laevis appears much lower. These results strongly suggest that animal vertebrate mitochondrial DNAs have followed the same evolutionary pathway.

【 授权许可】

Unknown