Cobamide‐containing membrane protein complex in Methanobacterium

FEBS Letters

Fuchs, Georg¹  Schulz, Harald¹ 

[1] Abteilung Angewandte Mikrobiologie, Universität Ulm, Oberer Eselsberg M 23, D-7900 Ulm, FRG

关键词: (Methanobacterium);    Methanogenesis;    Cobamide;    Corrinoid enzyme;    Vitamin B12;    Membrane protein;   

DOI  :  10.1016/0014-5793(86)80420-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Methanobacterium thermoautotrophicum, most of the sole cellular cobamide, 5-hydroxybenzimidazolylcobamide (factor III [(1986) Anal. Biochem., submitted]) is bound to a membrane protein. This corrinoid protein is solubilized as a protein complex of M_r ~500 000 by 5% nonpolar detergents. The complex is stable in 7 M urea. In SDS, two smaller fractions of M_r ~ 30 000 and 60 000 carrying the cobamide are found. The unusual properties of the corrinoid enzyme complex suggest a novel function in the metabolism of CO₂ and Hz in methanogens.

【 授权许可】

Unknown