Structural relationships between the NADH dehydrogenases of Paracoccus denitrificans and bovine heart mitochondria as revealed by immunological cross‐reactivities

FEBS Letters

Ferguson, Stuart J.¹  George, Christina L.¹  lan Ragan, C.²  Cleeter, Michael W.J.² 

[1] >Department of Biochemistry, University of Birmingham, PO Box 363, Birmingham BI5 27T England;Department of Biochemistry, University of Southampton, Southampton S09 3TU, England

关键词: NADH dehydrogenase;    (Paracoccus denitrificans);    Immmoblotting;    Respiratory chain;    Mitochondria;   

DOI  :  10.1016/0014-5793(86)81199-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An antibody raised against two subunits (M _r, 48000 and 25000) of NADH dehydrogenase from Paracoccus denitrificans cross-reacts with one of more than 20 polypeptides that form the bovine heart mitochondrial NADH dehydrogenase. The cross-reacting subunit has M _r, 51000 and is believed to be the NADH-binding subunit of the enzyme. Antibodies raised against certain subunits of the bovine heart NADH dehydrogenase were tested for cross-reactivity withP. denitrificans cytoplasmic membranes. Of those tested, only one, an antibody specific for the 49 kDa subunit of mitochondrial NADH dehydrogenase, cross-reacted with the bacterial membranes. It recognised a polypeptide of approximate M _r, 46000. This is an indication for a previously undetected third subunit of NADH dehydrogenase from P. denitrificans. The immunological crossreactions indicate that the NADH dehydrogenases from P. denitrificans and bovine heart mitochondria are related structurally.

【 授权许可】

Unknown