gp 140, the C3d/EBV receptor (CR2), is phosphorylated upon in vitro activation of human peripheral B lymphocytes

FEBS Letters

Barel, Monique²  Vazquez, Aimé¹  Galanauc, Pierre¹  Aufredou, Maria Thérèse¹  Charriaut, Christiane²  Frade, Raymond² 

[1] Université de Paris-Sud, INSERM U.131, Clamart, France;Laboratoire de Biochimie des Antigènes de Membrane, INSERM U.I 39, Hôpital H. Mondor, 94010 CreteilFrance

关键词: Complement receptor Virus receptor C3d Epstein-Barr virus Phosphorylation B lymphocyte Lymphocyte activation;    gp 140;    140 kDa membrane glycoprotein;    C3;    native third component of complement;    C3b;    175 kDa fragment of trypsin cleaved C3;    C3d;    the 33kDa fragment of C3;    EBV;    Epstein-Barr virus;    CR2;    C3 receptor type 2;    B cell receptor specific for C3d;    C3dg;    C3bi ⪢ C3b;    BCGF;    B cell growth factor;    SAC;    Staphylococcus aureus of the Cowan I strain;    anti-μ Ab;    anti IgM antibodies;    sIgG;    surface immunoglobulins;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    NP-40;    Nonidet P-40;   

DOI  :  10.1016/0014-5793(86)80356-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

gp 140, the C3d/EBV receptor (CR2), is a specific marker of human B lymphocytes. Very recent data suggest that CR2 is a membrane site involved in early B cell activation. These properties of CR2 led us to analyze the molecular events associated with gp 140. We analyzed whether in some conditions of B lymphocyte activation, CR2 could be phosphorylated. We have found that when highly enriched peripheral B cells were cultured for 48 h with anti-μ Ab and/or SAC, in order to provide an optimal activating signal, phosphorylation of the CR2 was induced.

【 授权许可】

Unknown