EGF and insulin action in fibroblasts

FEBS Letters

L'Allemain, Gilles¹  Pouysségur, Jacques¹ 

[1] Centre de Biochimie, CNRS, Parc Valrose, 06034 Nice, France

关键词: α-Thrombin Phosphoinositide cycle DNA synthesis Fibroblast Epidermal growth factor;   

DOI  :  10.1016/0014-5793(86)80354-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chinese hamster lung fibroblasts (CHL) arrested in G₀ by serum starvation reinitiate DNA synthesis in response to either EGF, thrombin or serum. Arrested cells, prelabelled to equilibrium with [³H]inositol and receiving 20 mM LiCl prior stimulation, released rapidly large amounts of inositol phosphates when stimulated with thrombin or serum. In sharp contrast, EGF alone, or in association with insulin, failed to induce phosphoinositide breakdown at either early or late stages of EGF stimulation or in growing cells in EGF-supplemented serum-free medium. Phospholipase C remained, however, highly activatable by thrombin at all stages of EGF stimulation. Since EGF and thrombin are equally potent mitogens for CHL, we conclude that hydrolysis of polyphosphoinositides is not an exclusive signalling pathway for commitment to DNA replication and cell division.

【 授权许可】

Unknown