Microsomal membrane subfractionation by a lectin affinity method

FEBS Letters

Williams, L.G.¹  Sehmi, D.¹  Williams, D.J.¹ 

[1] Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, England

关键词: Concanavalin A Immobilized lectin Microsomal subfractionation Plasma membrane;   

DOI  :  10.1016/0014-5793(86)80352-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Concanavalin A-agarose treatment of rat liver post-mitochondrial supernatant removes a fraction rich in cholesterol and 5'-nucleotidase activity but low in glucose-6-phosphatase. At the same time, radiolabel associated with the cell surface is removed. We interpret these findings as evidence that concanavalin A binds to, and under these circumstances will remove, fragments of plasma membrane present in the microsomal fraction and believe that this may be of use in the gentle, and rapid subfractionation of microsomal membranes.

【 授权许可】

Unknown