Characterization of furosemide‐sensitive Mg2+ influx in Yoshida ascites tumor cells

FEBS Letters

Averdunk, R.¹  Vormann, J.²  Günther, T.² 

[1] Institute of Clinical Chemistry and Clinical Biochemistry, Free University of Berlin. Hindenburgdamm 30, D-1000 Berlin 45, Germany;Institute of Molecular Biology and Biochemistry, Free University of Berlin, Arnimallee 22, D-1000 Berlin 33 Germany

关键词: Mg2+ influx Furosemide sensitivity (Yoshida ascites tumor cell);    2-DOG;    2-deoxyglucose;    DIDS;    4;    4'-diisothiocyanostilbene-2;    2'-disulfonic acid;    SITS;    4-acetamido-4'-isothiocyanostilbene-2;    2'-disulfonic acid;    [Mg2+]i;    intracellular Mg2+ content;    [Mg2+]o;    extracellular Mg2+ concentration;   

DOI  :  10.1016/0014-5793(86)80345-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Partially Mg²⁺-depleted Yoshida ascites tumor cells took up Mg²⁺ after reincubation in Mg²⁺-and HCO₃ ⁻-containing media. Mg²⁺ influx was insensitive to ouabain, amiloride and disulfonic stilbenes, but was noncompetitively inhibited by furosemide (K _i = 0.4 mM) and bumetanide. Mg²⁺ influx obeyed Michaelis-Menten kinetics with respect to Mg²⁺ concentration (K _m = 1.1 mM) and was sigmoidal with respect to HCO₃ ⁻ concentration. Electroneutral Mg²⁺, HCO₃ ⁻ cotransport was supposed to be the mechanism of Mg²⁺ influx.

【 授权许可】

Unknown