Crystallization and spectroscopic investigation with polarized light of the reaction center‐B875 light‐harvesting complex of Rhodopseudomonas palustris

FEBS Letters

Kreutz, Werner¹  Drews, Gerhart²  Becker, Andreas¹  Gad'on, Nasser²  Mäntele, Werner¹  Wacker, Thomas¹  Welte, Wolfram¹ 

[1] Institut für Biophysik und Strahlenbiologie, Albertstr. 23 Freiburg, FRG;Institut für Biologie II, Mikrobiologie, Schänzlestr. 1, 78 Freiburg, FRG

关键词: (Rhodopseudomonas palustris) Reaction center-B875 complex Membrane protein crystallization Linear dichroism;   

DOI  :  10.1016/0014-5793(86)80340-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A pigment-protein complex containing a reaction center and the B875 light-harvesting complex from Rhodopseudomonas palustris was purified and crystallized in the presence of detergent. Thin, rectangular crystals were obtained and used for optical spectroscopy. The protein pattern as well as the spectrum of the solubilized and crystallized complex show that the purified complex was crystallized without a major change in its composition and conformation. The absorption band intensities in the plane of the crystal as well as the spectra of the tilted crystal indicate that the 590 nm Q_x transitions are aligned along the long axis of the crystal, while the 875 nm Q_y transitions are aligned along the short axis of the crystal plane. The results favor a model where the antenna bacteriochlorophylls associated with one reaction center have a common Q_x direction and perpendicular to it a distribution of 875 nm Q_y transitions exhibiting a maximum along one direction.

【 授权许可】

Unknown