Electron microscopy of the hydrogenase from the hydrogen‐oxidizing bacterium Alcaligenes eutrophus Z1

FEBS Letters

Egorov, A.M.²  Popov, V.O.³  Utkin, I.B.²  Kiselev, N.A.¹  Berezir, I.V.³  Tsuprun, V.L.¹ 

[1] A.V. Shubnikov Institute of Crystallography, USSR Academy of Sciences, Moscow 117333, USSR;Department of Chemistry, Lomonosov Moscow State University, Moscow 117234, USSR;A.N. Bach Institute of Biochemistry, USSR Academy of Sciences, Moscow 117071, USSR

关键词: Hydrogenase Enzyme structure Electron microscopy;   

DOI  :  10.1016/0014-5793(86)80331-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The quaternary structure of the NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1 has been studied by electron microscopy. A model of the hydrogenase molecule with two small subunits arranged between two large ones in linear fashion is proposed. The electron microscopy studies are in accordance with the previously proposed linear electron transport chain of the hydrogenase and distribution of the substrate binding sites within the enzyme molecule.

【 授权许可】

Unknown