The N‐terminal 21 amino acids of a 70 kDa protein of the yeast mitochondrial outer membrane direct E. coli β‐galactosidase into the mitochondrial matrix space in yeast cells

FEBS Letters

Hase, Toshiharu¹  Nakai, Masato¹  Matsubara, Hiroshi¹ 

[1] Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan

关键词: Mitochondrial protein import (Yeast) Mitochondrial outer membrane Fusion protein Membrane protein Membrane binding;    N-;    amino-;    ONPG;    O-nitrophenyl galactoside;   

DOI  :  10.1016/0014-5793(86)80326-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intracellular location of fusion proteins was investigated in yeast cells. They consisted of the N-terminal 21,61 or 292 amino acids of the 70 kDa protein of the yeast mitochondrial outer membrane and an enzymatically active E. coli β-galactosidase. The hybrids containing 61 or 292 residues of the 70 kDa protein, as well as the original 70 kDa protein, were localized on the outer membrane in a tightly membrane-bound form. In contrast, the other hybrid was exclusively localized in the mitochondrial matrix space as a soluble protein.

【 授权许可】

Unknown