FEBS Letters | |
The actin‐activated ATPase of regulated and unregulated scallop heavy meromyosin | |
Jackson, Andrew P.1  Wells, Christine1  Warriner, Karen E.1  Bagshaw, Clive R.1  | |
[1] Department of Biochemistry. University of Leicester, Leicester LEI 7RH, England | |
关键词: ATPase Heavy meromyosin Myosin-linked regulation Ca2+ activation; | |
DOI : 10.1016/0014-5793(86)80317-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Single-turnover kinetic analysis indicates that scallop heavy meromyosin (HMM) preparations contain a small fraction of unregulated molecules which dominate the steady-state ATPase activity in the absence of Ca2+. This fraction was removed by rapid centrifugation during an effective single turnover of the ATPase in the presence of actin. The maximum ATPase activity of a scallop myosin head was estimated as 10 s−1 by cross-linking subfragment 1 to F-actin. HMM became Ca2+-insensitive during the cross-linking procedure. Attachment of spectroscopic probes to the reactive thiol group of scallop HMM resulted in the retention of ATPase activity but a loss in Ca2+ sensitivity.
【 授权许可】
Unknown
【 预 览 】
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