A chemiluminescent probe with a Cypridina luciferin analog, 2‐methyl‐6‐phenyl‐3,7‐dihydroimidazo[1,2‐a]pyrazin‐3‐one, specific and sensitive for O2 − production in phagocytizing macrophages

FEBS Letters

Kurashige, Satonori¹  Sugioka, Katsuaki¹  Akuzawa, Yuki¹  Nakano, Minoru¹  Goto, Toshio² 

[1] College of Medical Care and Technology, Gunma University, Maebashi, Gunma 371 Japan;Laboratory of Organic Chemistry, Faculty of Agriculture, Nagoya University, Chikusaku, Nagoya 464, Japan

关键词: Macrophage Superoxide anion Cypridina Luciferin analog Chemiluminescence;   

DOI  :  10.1016/0014-5793(86)80291-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When a Cypridina luciferin analog (the title compound) was added to a macrophage suspension in Hank's balanced salt solution (control), the system emitted a weak, but detectable light, which was not altered in the presence of Superoxide dismutase. The same system, however, emitted a much stronger light, just after the addition of a trigger, opsonized zymosan. The luminescence was suppressed to the control level in the presence of superoxide dismutase, while it was only slightly influenced, if at all, by NaN₃, a scavenger of singlet oxygen and an inhibitor of myeloperoxidase. Some other results obtained also indicate the participation of O₂ ⁻ in the luciferin analog-dependent luminescence in macrophages during phagocytosis.

【 授权许可】

Unknown