FEBS Letters | |
A chemiluminescent probe with a Cypridina luciferin analog, 2‐methyl‐6‐phenyl‐3,7‐dihydroimidazo[1,2‐a]pyrazin‐3‐one, specific and sensitive for O2 − production in phagocytizing macrophages | |
Kurashige, Satonori1  Sugioka, Katsuaki1  Akuzawa, Yuki1  Nakano, Minoru1  Goto, Toshio2  | |
[1] College of Medical Care and Technology, Gunma University, Maebashi, Gunma 371 Japan;Laboratory of Organic Chemistry, Faculty of Agriculture, Nagoya University, Chikusaku, Nagoya 464, Japan | |
关键词: Macrophage Superoxide anion Cypridina Luciferin analog Chemiluminescence; | |
DOI : 10.1016/0014-5793(86)80291-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
When a Cypridina luciferin analog (the title compound) was added to a macrophage suspension in Hank's balanced salt solution (control), the system emitted a weak, but detectable light, which was not altered in the presence of Superoxide dismutase. The same system, however, emitted a much stronger light, just after the addition of a trigger, opsonized zymosan. The luminescence was suppressed to the control level in the presence of superoxide dismutase, while it was only slightly influenced, if at all, by NaN3, a scavenger of singlet oxygen and an inhibitor of myeloperoxidase. Some other results obtained also indicate the participation of O2 − in the luciferin analog-dependent luminescence in macrophages during phagocytosis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020287732ZK.pdf | 376KB | download |