Thyrotropin and cyclic AMP regulation of ras proto‐oncogene expression in cultured thyroid cells

FEBS Letters

Hirayu, Hideshi²  Rapoport, Basil¹  Dere, Willard H.² 

[1] Departments of Medicine, University of California, San Francisco, CA 94143, USA;Departments of Medicine, Veterans' Administration Medical Center, Metabolism (111F), San Francisco, CA 94121 USA

关键词: Thyroid Thyrotropin Oncogene cyclic AMP;   

DOI  :  10.1016/0014-5793(86)80268-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined the effect of thyrotropin (TSH) on intracellular levels of c-ras mRNA in a line of differentiated rat thyroid cells obtained from normal Fischer rat thyroids. These cells are totally dependent on TSH for growth. TSH stimulation of quiescent cells increased c-ras mRNA content, with a maximal response (730% of basal) after 6 h, and a decline towards basal levels after 24 h. Dibutyryl cAMP and forskolin mimicked this stimulatory effect of TSH on c-ras, but did not enhance β-actin mRNA content. This study demonstrates hormonal and cyclic nucleotide control of c-ras expression in a well-differentiated, non-tumorogenic mammalian cell.

【 授权许可】

Unknown