Vasopressin‐stimulated phosphorylation of rat liver phospholipid methyltransferase in isolated hepatocytes

FEBS Letters

Mérida, Isabel¹  Cabrero, Carmen¹  Mato, José M.¹  Alvarez, José F.¹  Varela, Isabel¹ 

[1]Metabolismo, Nutrición y Hormonas, Fundación Jiménez Díaz, Reyes Católicos 2, 28040 Madrid, Spain

关键词: Vasopressin Phospholipid methyltransferase Protein phosphorylation Electroelution Hepatocyte Phosphoamino acid;   

DOI  :  10.1016/0014-5793(86)80262-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of vasopressin (1μM) to isolated rat hepatocytes prelabeled with [³²P]phosphate was accompanied by a 250% increase in the phosphorylation of phospholipid methyltransferase. Vasopressin-stimulated phospholipid methyltransferase phosphorylation was time- and dose-dependent. ³²P-labeled phospholipid methyltransferase was recovered by immunoprecipitation and SDS-polyacrylamide gel electrophoresis. After electrophoresis, phospholipid methyltransferase was electroeluted from the polyacrylamide gel and subjected to tryptic digestion or HCl hydrolysis. Analysis of ³²P-labeled peptides reveals only one site of phosphorylation and the analysis of [³²P]phosphoamino acids indicates that phosphoserine is the only labeled amino acid.

【 授权许可】

Unknown