The photochemical cycle of bacteriorhodopsin has no refractory period

FEBS Letters

Oesterhelt, D.²  Tittor, J.²  Dancshazy, Z.¹  Groma, G.I.¹ 

[1] Institute of Biophysics, Biological Research Center of Hungarian Academy of Science, POB 521, Szeged, Hungary;Max-Planck-Institute for Biochemistry, D-8033 Martinsried, FRG

关键词: Bacteriorhodopsin;    Flash absorption spectroscopy;    Photocycle;    Catalytic cycle;    Double-pulse excitation;   

DOI  :  10.1016/0014-5793(86)80245-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Double-pulse experiments were used to probe the existence of a refractory period in the photochemical cycle of bacteriorhodopsin. We demonstrate using three different approaches that no refractory period exists in purple membranes, cell vesicles or intact cells. First, we showed by applying different time delays between two flashes that every molecule which regained the initial optical state could immediately recycle again. Second, we found no difference in the effectiveness of the first and second flash when the intensity was varied. Third, different steady-state levels of the intermediates were produced with different intensities of continuous background illumination. No difference in effectivity of the first and second flash could be detected.

【 授权许可】

Unknown