Thermodynamics of left‐handed helix formation

FEBS Letters

Klump, H.H.¹ 

[1] Institut für Physikalische Chemie, Universität Freiburg, Albertstr. 23a, D-7800 Freiburg, FRG

关键词: Enthalpy;    Helix formation;    Left-handed helix;    Calorimetry;    Entropy;   

DOI  :  10.1016/0014-5793(86)80237-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thermodynamics of right- and left-handed helix formation by poly[d(G-C)]·poly[d(G-C)] and by poly-(dG-m⁵dC)·poly(dG-m⁵dC) were measured spectrophotometrically and calorimetrically. From the spectrophotometric measurements the thermal stabilities of the alternative helical conformations were evaluated as a function of counterion concentration. From the calorimetric measurements the enthalpies of either right-handed or left-handed helix formation were determined. The corresponding experimental ΔH values are −8.6 and −11.2 base pairs for the two conformations in poly[dG-C)]·poly[d(G-C)], and −9.0 and −12.7 base pairs, respectively, for poly(dG-m⁵dC)·poly(dG-m⁵dC).

【 授权许可】

Unknown