Sequence analysis of bovine retinal S‐antigen

FEBS Letters

Craft, Cheryl¹  Katial, Albine¹  Wistow, Graeme J.¹  Shinohara, Toshimichi¹ 

[1] Laboratory of Molecular and Developmental Biology, NEI Bldg 6, Room 204, National Institutes of Health, Bethesda, MD 20892, USA

关键词: (Bovine retina);    S-Antigen;    Uveitis;    cDNA sequence;    α-Transducin;    ADP-ribosylation;   

DOI  :  10.1016/0014-5793(86)80207-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

S-Antigen is a major soluble protein of the retina and pineal. It is capable of inducing experimental autoimmune uveitis (EAU) in laboratory animals and also seems to play an important role in the visual cycle. The results of partial cDNA sequence analysis reveal interesting homologies with a-transducin, a GTP-binding protein of retina and other purine nucleotide-binding proteins. In particular S-antigen shows over 50% identity to the proposed pertussis toxin ADP-ribosylation site of α-transducin. It also contains the Gly-X-X-X-X-Gly-Lys pattern common to phosphoryl binding sites. A possible relationship between S-antigen and purine nucleotide-binding proteins is discussed. There is also evidence for a repetitious β-structure in the C-terminal half of S-antigen, with a monoclonal antibody epitope in a helical region at the C-terminus.

【 授权许可】

Unknown