Characterization and quantitative topographical distribution of salmon calcitonin‐binding sites in rat kidney sections

FEBS Letters

Milhaud, G.¹  Bouizar, Z.¹  Moukhtar, M.S.¹  Rostène, W.H.² 

[1] UA 163 CNRS and U 113 INSERM, Service de Biophysique, Faculté de Médecine Saint-Antoine, 27 rue Chaligny, 75571 Paris Cedex 12, France;INSERM U 55, Hôpital Saint-Antoine, 184 rue du Faubourg Saint-Antoine, 75012 Paris, France

关键词: Calcitonin localization;    Autoradiography;    Binding site;    (Kidney section;    Rat);    pCT;    synthetic pig CT;    hCT;    synthetic human CT;    VIP;    vasoactive intestinal peptide;    1–34 PTH;    parathyrine;    TRH;    thyrotropin-releasing hormone;    CCK;    cholecystokinin-8;    Met-Enk;    met-enkephalin;    NT;    neurotensin;   

DOI  :  10.1016/0014-5793(86)80206-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Renal binding sites for labelled salmon calcitonin (sCT) were studied using cryostat sections and autoradiography. Increasing concentrations of unlabelled sCT inhibited ¹²⁵I-sCT binding. ¹²⁵I-sCT bound to a single site with a K _d of 2 nM and a number of sites of 220 protein. Mammalian calcitonins had low affinities and peptides unrelated to CT were devoid of any significant affinity for ¹²⁵I-sCT receptors. Auto-radiograms disclosed a high concentration of ¹²⁵I-sCT receptors mainly located in the outer medulla and heterogeneously in the renal cortex. The distribution of specific binding sites is in agreement with the current concepts of renal action of calcitonin.

【 授权许可】

Unknown