Proliferating and quiescent cells exhibit different subcellular distribution of protein kinase C activity

FEBS Letters

Lazdins, J.¹  Caporale, C.²  Nervi, C.²  Aguanno, S.²  Molinaro, M.²  Cortesi, E.¹  Gastaldi, R.²  Adamo, S.²  Faggioni, A.¹  Belli, L.¹ 

[1]Istituto di Patologia Generale, Universitá di Roma ‘La Sapienza’, via Scarpa 14, 00161 Rome, Italy

[2]Istituto di Istologia ed Embriologia Generale, Universitá di Roma ‘La Sapienza’, via Scarpa 14, 00161 Rome, Italy

关键词: Protein kinase C Cell proliferation Phospholipid Diacylglycerol Phorbol ester;   

DOI  :  10.1016/0014-5793(86)80192-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activity of calcium, phospholipid-dependent protein kinase (PKc), which is thought to play an important role in cell proliferation, has been measured in the particulate and soluble fractions of cultured cells, under different proliferative conditions. Our results indicate that proliferating cells display higher PKc activity than quiescent cells. Furthermore, in both normal and transformed cells, PKc is preferentially associated with the particulate fraction when the cells are proliferating, while in mitotically quiescent cells the majority of the enzyme activity is found in the soluble fraction. These data suggest that PKc activity and subcellular distribution undergo spontaneous changes according to the proliferative state of the cells.

【 授权许可】

Unknown