The diurnal rhythm of liver glycogen phosphorylase: correlating changes in enzyme activity and enzymic protein

FEBS Letters

Roesler, William J.¹  Khandelwal, Ramji L.¹ 

[1] College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada

关键词: Diurnal rhythm (Mouse liver) Glycogen phosphorylase Glycogen Rocket immunoelectrophoresis;   

DOI  :  10.1016/0014-5793(86)80190-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The diurnal rhythm of phosphorylase activity in mouse liver extracts was correlated with the 24 h fluctuations in phosphorylase protein. This last measurement was made using rocket immunoelectrophoresis. The peak activity of phosphorylase appeared coincident (at 18:00 h) with the greatest amount of phosphorylase protein detected. Conversely, the lowest activity measured and lowest enzymic protein content both occurred at 02:00 h. Regression analysis revealed a significant positive correlation between enzyme activity and protein. Thus changes in the cellular concentration of this enzyme are implicated in the diurnal rhythm of liver glycogen.

【 授权许可】

Unknown