Effect of 2,4‐dinitrofluorobenzene on the enzymatic properties of the b‐c 1 complex isolated from beef heart mitochondria

FEBS Letters

Gatti, D.¹  Lorusso, M.¹  Marzo, M.¹  Papa, S.¹ 

[1] Institute of Medical Biochemistry and Chemistry, University of Bari, Bari, Italy

关键词: Cytochrome b-C1 complex Chemical modification Amino acid residue Antimycin binding;    DNFB;    2;    4-dinitrofluorobenzene;    SDSPAGE;    SDS-polyacrylamide gel electrophoresis;    DQH2;    durohydroquinone;   

DOI  :  10.1016/0014-5793(86)80181-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A study is presented on the effect of 2,4-dinitrofluorobenzene (DFNB) on the enzymatic properties of mitochondrial b-c ₁ complex. The chemical modification by DNFB strongly inhibits the reductase activity of the complex, this being accompanied by labelling by [³H]DNFB of core protein I, the apoprotein of b cytochromes and the 12 kDa subunit. Chemical modification by DNFB appears to alter, in particular, the domain of heme b-562.

【 授权许可】

Unknown