Do the higher oxidation states of the photosynthetic O2‐evolving system contain bound H2O?

FEBS Letters

Radmer, Richard¹  Ollinger, Otto¹ 

[1] Martin Marietta Laboratories, 1450 South Rolling Road, Baltimore, MD 21227, USA

关键词: Photosynthesis Photosystem II Oxygen evolution Mass spectrometry 18O;   

DOI  :  10.1016/0014-5793(86)80178-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A modified mass spectrometer was used to determine whether the higher oxidation states of the photosynthetic O₂-evolving system contain substrate water that is not freely exchangeable with the external medium. Our data indicated that the higher oxidation states contain no appreciable bound, non-exchangeable H₂O. This suggests that H₂O oxidation takes place via a rapid, concerted, all-or-none mechanism rather than by a mechanism involving stable, partially oxidized, H₂O-derived intermediates. These findings set definite constraints on possible mechanisms of O₂ evolution.

【 授权许可】

Unknown