Stimulation of estrogen receptor accumulation by estradiol in primary cultures of salmon hepatocytes

FEBS Letters

Lazier, Catherine B.¹  Mommsen, Thomas P.¹ 

[1] Departments of Biology and Biochemistry Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada

关键词: (Salmon hepatocyte culture) Estrogen receptor Estradiol;   

DOI  :  10.1016/0014-5793(86)80174-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatocytes of Salmo salar in primary culture form confluent monolayers and can be maintained at 11 °C in serum-free medium for 8 days with minimal cell loss. Cultured hepatocytes from immature male salmon contain estrogen receptor both in nuclear and cytosol fractions (2000 and 2400 sites/cell, respectively). A single addition of estradiol results in an increase in the nuclear receptor to a level of 23 000 sites/cell after 24 h. This nuclear receptor concentration is similar to that in liver of estrogen-treated salmon in vivo, and is much higher than has been found for any other egg-laying vertebrate. The cultured salmon hepatocytes thus represent a highly sensitive system for the study of estrogen receptor dynamics and vitellogenesis in vitro.

【 授权许可】

Unknown