Application of monoclonal antibodies to the isolation and characterization of a killer toxin secreted by Hansenula mrakii

FEBS Letters

Tachibana, Katsumi¹  Imai, Mitsunobu¹  Mayumi, Makoto¹  Yamamoto, Tetsuro¹ 

[1] Immunology Division, Jichi Medical School, Minamikawachi-Machi, Tochigi-Ken 329-04 and Japanese Red Cross Blood Center at Saitama-Ken 367, Japan

关键词: Killer toxin (Hansenula mrakii) Monoclonal antibody Affinity chromatography Amino acid sequence;   

DOI  :  10.1016/0014-5793(86)80170-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae. Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic polypeptide with an isoelectric point at pH 9.1, and devoid of mannosides. It is composed of 88 amino acid residues with a molecular size of 10721 Da. The monoclonal antibodies could be applicable to the analysis of biologically active sites on the toxin, in an attempt to synthesize chemically a small peptide with killer activity and little immunogenicity.

【 授权许可】

Unknown