The GTP‐binding regulatory proteins of neuroblastoma × glioma, NG108‐15, and glioma, C6, cells

FEBS Letters

Klee, Werner A.¹  Spiegel, Allen M.¹  Gierschik, Peter²  Milligan, Graeme¹ 

[1] Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892, USA;Metabolic Diseases Branch, National Institute of Arthritis, Diabetes, Digestive and Kidney Disease, National Institute of Health, Bethesda, MD 20892, USA

关键词: GTP-binding protein Immunoblotting Pertussis toxin ADP-ribosylation;   

DOI  :  10.1016/0014-5793(86)80165-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amounts of the guanine nucleotide binding regulatory proteins which are also pertussis toxin substrates (such as N_i and N_o) were measured in rat glioma, C6BU-1, cells and in neuroblastoma × glioma, NG108-15, hybrid cells. Measurements were performed both by quantitating pertussis toxin catalyzed ADP-ribosylation and by quantitative immunoblotting with affinity purified antibodies specific for N_i or N_o. The amounts of pertussis toxin substrate in C6 and NG108-15 cells are 7.5 and 0.6 membrane protein, respectively. These levels are minimum values and higher estimates of the total amounts of N proteins in the two cells are obtained by quantitative immunoblot analysis of the β-subunit common to all N proteins. Immunoblots with specific antibodies show that NG108-15 cells contain 3.8 of N_o and detectable but small ( < 0.1 ) amounts of N_i. In contrast, C6 cell membranes contain no detectable N_o and only 0.14 N_i. Thus, C6 cells contain large amounts of a pertussis toxin substrate which is neither N_i nor N_o.

【 授权许可】

Unknown