Degradation of oxalate in rats implanted with immobilized oxalate oxidase

FEBS Letters

Tarachand, U.¹  Raghavan, K.G.¹ 

[1] Biochemistry Division, Bhabha Atomic Research Centre, Bombay 400 085, India

关键词: Oxalate oxidase Nephrolithiasis Enzyme therapy Immobilized enzyme;   

DOI  :  10.1016/0014-5793(86)80139-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Accumulation of oxalate leads to hyperoxaluria and calcium oxalate nephrolithiasis in man. Since oxalate is a metabolic end product in mammals, the feasibility of its enzymic degradation has been tested in vivo in rats by administering exogenous oxalate oxidase. Oxalate oxidase, isolated from banana fruit peels, in its native form was found to be non-active at the physiological pH of the recipient animal. However, its functional viability in the recipient animal was ensured by its prior binding with ethylenemaleic anhydride, thus shifting its pH activity curve towards the alkaline range. Rats implanted with dialysis membrane capsules containing such immobilized oxalate oxidase in their peritoneal cavities effectively metabolized intraperitoneally injected [¹⁴C]oxalate as well as its precursor [¹⁴C]glyoxalate. The implantation of capsules containing coentrapped multienzyme preparations of oxalate oxidase, catalase and peroxidase led to a further degradation of administered [¹⁴C]oxalate in rats.

【 授权许可】

Unknown