Biochemical analysis of bovine testicular anti‐Müllerian hormone

FEBS Letters

Josso, Nathalie¹  Bourrillon, R.²  Picard, Jean-Yves¹  Goulut, Chantal² 

[1] Unité de Recherches sur l'Endocrinologie du Développement, INSERM, Hôpital des Enfants-Malades, 75743 Paris cedex 15, France;Département de Biochimie, UER des Saints-Péres, 45 rue des Saints-Pères, 75006 Paris, France

关键词: (Fetal testis) Anti-Müllerian hormone Mülllerian inhibiting substance Glycoprotein;   

DOI  :  10.1016/0014-5793(86)80133-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Direct biochemical analysis has been applied to bovine testicular anti-Müllerian hormone (AMH), purified from incubation medium of bovine fetal testes by immunochromatography on a monoclonal antibody. The hormone contains a high proportion of hydrophobic amino acids and 13.5% carbohydrate. The oligosaccharide composition suggests that both N- and O-glycosidically linked chains are present. The molecular extinction coefficient is 3.27 ± 0.06. One RIA unit, defined as the amount of hormone released by 1 g fetal bovine testicular tissue incubated during 4 h, corresponds to 3.06 ± 0.17 /smg protein.

【 授权许可】

Unknown