Cloning and DNA sequence of the 5'‐exonuclease gene of bacteriophage T5

FEBS Letters

Krutilina, A.I.¹  Kaliman, A.V.¹  Bayev, A.A.¹  Kryukov, V.M.¹ 

[1] Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Pushchino, Moscow region 142292, USSR

关键词: Bacteriophage T5 DNA sequencing D15 gene IS1 element;   

DOI  :  10.1016/0014-5793(86)80130-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of the BalI-PstI fragment of T5 DNA, 1347 bp in length, coding for 5'-exonuclease (D15 gene), has been determined. A coding region of the gene contains 873 bp and is preceded by a typical Shine-Dalgarno sequence. The D15 gene belongs to a cluster, consisting of at least 3 genes, in which a termination codon of a preceding gene overlaps an initiation codon of the following one. The sequence contains an open reading frame for 291 amino acid residues. The molecular mass of the 5'-exonuclease calculated from the predicted amino acid sequence is 33400 Da.

【 授权许可】

Unknown