Kinetics of electron transfer in reaction center‐cytochrome o proteoliposomes

FEBS Letters

Leslie Dutton, P.²  Robertson, Dan E.²  Moser, Christopher C.²  Kaback, H.Ronald¹  Matsushita, Kazunobu¹ 

[1] Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110, USA;Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104 USA

关键词: Cytochrome o Reaction center Electron transfer Proteoliposome;   

DOI  :  10.1016/0014-5793(86)80061-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A proteoliposome hybrid protein system consisting of photosynthetic reaction centers (RC) from Rhodopseudomonas sphaeroides, cytochrome o from E. coli and ubiquinone-8 has been reconstituted to study the mechanism of O₂ reduction and the generation of Δs̃mH⁺ in cyt. o. Steady-state illumination leads to O₂ uptake and the generation of an interior negative ΔΨ. A rapid flash of light induces a single turnover of each RC, and the resultant pulse of quinol leads to quinonoid inhibitor-sensitive millisecond reduction kinetics and cyanide-sensitive re-oxidation kinetics of cyt o. The b hemes are similar on a kinetic, redox and α-band spectral basis.

【 授权许可】

Unknown