A calcium‐protease activator associated with brain microsomal‐insoluble elements

FEBS Letters

Kishimoto, Akira¹  Nakanishi, Hiroyuki¹  Takeyama, Yoshihumi¹  Uratsuji, Yuko¹  Nishizuka, Yasutomi¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

关键词: Calpain Calpain activator Protease;   

DOI  :  10.1016/0014-5793(86)80060-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A factor which markedly activates Ca²⁺-dependent thiol protease (calpain) is associated with Triton X-100-insoluble materials, presumably structural elements such as cytoskeletons, of bovine brain microsomal fraction. This factor is extracted with 0.6 M KCl, and purified partially by sucrose density gradient centrifugation and hydroxyapatite column chromatography. The factor appears to be a heat-stable protein with an approximate M _r, of 15000. With casein as substrate this factor activates both calpain I and calpain II severalfold up to more than 10- fold without alteration of their affinity to Ca²⁺. Calmodulin is unable to substitute for this factor. A similar factor is associated with human platelet insoluble materials.

【 授权许可】

Unknown