Kinetics of 125I‐ubiquitin conjugation with and liberation from rabbit reticulocyte stroma

FEBS Letters

Dubiel, W.¹  Müller, M.¹  Rapoport, S.¹ 

[1]Institute of Physiological and Biological Chemistry, Humboldt University Berlin, DDR- 1040 Berlin, GDR

关键词: Ubiquitin conjugation Reticulocyte stroma ATP-dependent proteolysis;   

DOI  :  10.1016/0014-5793(86)80049-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The breakdown of mitochondria-containing stroma of rabbit reticulocytes is an ATP- and ubiquitin-dependent process and there is no evidence for an ATP-dependent but ubiquitin-independent proteolysis in these cells. The ubiquitin conjugate formation with heat-denatured stroma proteins is about one-fifth of that with native stroma. In reticulocytes there exist two mechanisms of ubiquitin liberation from its conjugates with stroma proteins: (i) an ATP-dependent and hemin-resistant release of ubiquitin, which is assumed to be the first step in the degradation of ubiquitin conjugates by the protease system, and (ii) a release of ubiquitin catalyzed by an isopeptidase activity.

【 授权许可】

Unknown