ATP‐dependent translocation of amino phospholipids across the human erythrocyte membrane

FEBS Letters

Tilley, Leann¹  Roelofsen, Ben¹  den Kamp, Jos A.F.Op¹  van Deenen, Laurens L.M.¹  Cribier, Sophie² 

[1] Laboratory of Biochemistry, State University of Utrecht, Padualaan 8, NL-3584 CH Utrecht, The Netherlands;Institut de Biologie Physio-Chimique, 13, Rue Pierre et Marie Curie, F-75005 Paris, France

关键词: Erythrocyte membrane Phospholipid distribution Transbilayer mobility Lipid transfer protein Phospholipase ATP depletion;   

DOI  :  10.1016/0014-5793(86)80044-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trace amounts of radiolabeled phospholipids were inserted into the outer membrane leaflet of intact human erythrocytes, using a non-specific lipid transfer protein. Phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine were transferred from the donor lipid vesicles to the membrane of the intact red cell with equal ease, whilst sphingomyelin was transferred 6-times less efficiently. The transbilayer mobility and equilibrium distribution of the labeled phospholipids were assessed by treatment of the intact cells with phospholipases. In fresh erythrocytes, the labeled amino phospholipids appeared to move rapidly towards the inner leaflet. The choline phospholipids, on the other hand, approached an equilibrium distribution which strongly favoured the outer leaflet. In ATP-depleted erythrocytes, the relocation of the amino phospholipids was markedly retarded.

【 授权许可】

Unknown