Multiple relationships between rate of oxidative phosphorylation and Δµ̃H in rat liver mitochondria

FEBS Letters

Zoratti, Mario¹  Petronilli, Valeria¹ 

[1] CNR Unit for the Physiology of Mitochondria and Institute of General Pathology, Via Loredan 16, 35131 Padova, Italy

关键词: Oxidative phosphorylation;    Electrochemical proton gradient;    (Rat liver mitochondria);    Energy transduction;    Flow-force relationship;    Δµ̃H;    transmembrane electrochemical proton gradient;    Δψ;    transmembrane electrical potential difference;    J atp;    rate of ATP synthesis;    J o;    rate of oxygen consumption;    TPMP;    triphenylmethylphosphonium;    DMO;    5;    5-dimethyloxazolidine-2;    4-dione;    G6P;    glucose 6-phosphate;    Mops;    4-morpholinepropanesulfonic acid;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    RLM;    rat liver mitochondria;   

DOI  :  10.1016/0014-5793(85)80168-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relationship between rate of ATP synthesis and transmembrane electrochemical proton gradient has been determined in rat liver mitochondria oxidizing succinate, using the respiratory inhibitor malonate or the uncoupler FCCP to decrease Δµ̃H progressively. As previously reported [(1982) Eur. J. Biochem. 126, 443-451] two different relationships are obtained depending on the method used. Evidence is presented that this result is not due to underestimation of the Δµ̃H maintained by fast-respiring mitochondria, as recently suggested [(1985) FEBS Lett. 181, 323-327].

【 授权许可】

Unknown