Tenacious binding of lipids to vimentin during its isolation and purification from Ehrlich ascites tumor cells

FEBS Letters

Scherbarth, Annemarie¹  Perides, Georgios¹  Traub, Peter¹  Traub, Ulrike¹ 

[1] Max-Planck-Institut für Zellbiologie, Rosenhof, D-6802 Ladenburg/Heidelberg, FRG

关键词: Intermediate filament;    Vimentin;    Triton cytoskeleton;    Lipid;    (Ehrlich ascites tumor cell);   

DOI  :  10.1016/0014-5793(85)80155-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vimentin enriched in cytoskeletal frameworks by Triton X-100 extraction of Ehrlich ascites tumor cells and purified from a low ionic strength extract of the cell residues by (NH₄)₂SO₄ precipitation and DEAE-Sepharose and ssDNA-cellulose chromatography in the presence of 6 M urea was highly contaminated with lipids. Thin-layer chromatography of a chloroform-methanol extract of the purified protein revealed, besides small amounts of phospholipids, the presence of large quantities of neutral lipids.

【 授权许可】

Unknown