Glycerol stabilizes oxygen evolution and maintains binding of a 9 kDa polypeptide in photosystem II particles from the cyanobacterium, Phormidium laminosum

FEBS Letters

Stewart, Alison C.¹  Ljungberg, Ulf²  Siczkowski, Martin¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, England;Department of Biochemistry, University of Lund, Box 124, S-221 00 Lund, Sweden

关键词: Oxygen evolution;    Photosystem II;    Thermophilic cyanobacterium;    9 kDa polypeptide;    Glycerol requirement;    Hydrophobie interaction;    Mes;    4-morpholineethanesulfonic acid;    chl;    chlorophyll;    LiDS-PAGE;    lithium dodecyl sulfate polyacrylamide gel electrophoresis;    PS;    photosystem;   

DOI  :  10.1016/0014-5793(85)80145-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

High concentrations of glycerol (⩾20% ) stabilize oxygen evolution in photosystem (PS) II particles from the thermophilic cyanobacterium, Phormidium laminosum. Treating PS II particles with lower glycerol concentrations inhibits activity and, in parallel, detaches a 9 kDa polypeptide from PS II. These results suggest that the 9 kDa polypeptide is essential for PS II activity, and that it is bound to PS II primarily by hydrophobic interactions that are strengthened in vitro by glycerol. The 9 kDa polypeptide is distinct from cytochrome b-559 and does not appear to be associated with manganese or with the calcium or chloride requirements for oxygen evolution.

【 授权许可】

Unknown