Resistance to alloxan of tumoral insulin‐producing cells

FEBS Letters

Sener, Abdullah¹  Malaisse, Willy J.¹ 

[1] Laboratory of Experimental Medicine, Brussels Free University, Brussels B-1000, Belgium

关键词: Tumoral insulin-producing cell;    Pancreatic islet;    Alloxan;   

DOI  :  10.1016/0014-5793(85)80140-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat pancreatic islets and insulin-producing cells of the RINm5F line were incubated for 5 min at 7 or 23°C in media containing ³H₂O and either L-[1-¹⁴C]glucose or [2-¹⁴C]alloxan. In the islets the intracellular distribution space of [2-¹⁴C]alloxan represented, at 7 and 23°C respectively, 11.4 ± 1.0 and 25.5 ± 2.3% of the intracellular ³H₂O space. In the RINm5F cells, the distribution space of [2-¹⁴C]alloxan failed to be affected by the ambient temperature and represented, after correction for extracellular contamination, no more than 5.2 ± 0.5% of the intracellular ³H₂O space. Preincubation for 30 min at 7°C in the presence of alloxan (10 mM) failed to affect subsequent D-[U-¹⁴C]glucose oxidation in the tumoral cells, whilst causing a 70% inhibition of glucose oxidation in the islets. It is proposed that RINmSF cells are resistant to the cytotoxic action of alloxan, this being attributable, in part at least, to poor uptake of the diabetogenic agent.

【 授权许可】

Unknown