Monensin‐dependent and ‐independent mechanisms of cell‐matrix adhesion

FEBS Letters

Niven, Veronica M.¹  Aplin, John D.¹ 

[1] Department of Obstetrics and Gynaecology, University of Manchester, at St. Mary's Hospital, Whitworth Park, Manchester M13 0JH, England

关键词: Adhesion;    Extracellular matrix;    Monensin;    Surface labeling;    SCM;    subcellular matrix;    LPO;    lactoperoxidase;    HBSS;    Hank's balanced salt solution;   

DOI  :  10.1016/0014-5793(85)80138-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Attachment and spreading of human FL cells on a subcellular matrix (SCM) preparation made by treating confluent cell monolayers with deoxycholate are insensitive to the presence of monensin. However, if the cell suspension is surface-iodinated prior to adhesion using the LPO/H₂O₂ system, cell spreading on SCM is inhibited by 1 μM monensin. The suggested interpretation is that cell surface components required for cell spreading on SCM are inactivated by iodination and need replacement from intracellular reserves by a monensin-sensitive pathway. This pathway is not required in the absence of iodination when sufficient surface components (or a monensin-independent pathway of surface expression) are available. Support for this interpretation is obtained by means of double-iodination experiments in which surface-labelled cells adhere and spread, are detached and labelled a second time and then allowed to adhere again to SCM. Cell spreading in the second case is inhibited by ~ 80%, suggesting that both previously expressed and newly recruited receptors are inactivated.

【 授权许可】

Unknown