The determination of intracellular water space by NMR

FEBS Letters

Shinar, Hadassah¹  Navon, Gil¹ 

[1] School of Chemistry, Tel-Aviv University, Ramat-Aviv 69978, Tel Aviv, Israel

关键词: Intracellular water space;    2H-NMR;    59Co-NMR;    Erythrocyte;   

DOI  :  10.1016/0014-5793(85)80082-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of ⁵⁹Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN)₆ ³⁻ or Co(imidazole)₆ ³⁺ and the ²H or ¹H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured

【 授权许可】

Unknown