In vitro methylation of the elongation factor EF‐Tu from Escherichia coli

FEBS Letters

Jerez, Carlos A.¹  Toledo, Héctor¹ 

[1] Departamento de Bioquimica, División Ciencias Médicas Norte, Universidad de Chile, Casilla 70086, Santiago 7, Chile

关键词: Elongation factor EF-Tu;    Protein methylation;    DNA-dependent system;    Ethionine;    EF-Tu;    elongation factor Tu;    AdoMet;    S-adenosylmethionine;    Lys(Me);    ϵ-N-monomethyllysine;    Lys(Me2);    ϵ-N-dimethyllysine;    Lys(Me3);    ϵ-N-trimethyllysine;    M-His;    π-methylhistidine;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(85)80070-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in vitro methylation of the elongation factor EF-Tu from Escherichia coli was investigated. The methylation of newly synthesized EF-Tu was obtained using λrif^d18 DNA as template and S-adenosyl [methyl-³H]methionine as methyl donor. About 3 mol methyl residues were incorporated for every 10 mol EF-Tu synthesized. Analysis of the nature of the methyl-containing residues by protein hydrolysis followed by paper chromatography showed that both mono- and dimethyllysine were present. The methylation of EF-Tu was also studied separately from its synthesis by using cell-free systems with artificially undermethylated components.

【 授权许可】

Unknown