H+/ion antiport as the principal mechanism of transport systems in the vacuolar membrane of the yeast Saccharomyces carlsbergensis

FEBS Letters

Lichko, L.P.¹  Okorokov, L.A.¹  Polorotova, E.V.¹  Kulakovskaya, T.V.¹ 

[1] Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR

关键词: H+-ATPase;    Cation;    H+;    Antiporter;    Vacuolar membrane;    (Yeast);   

DOI  :  10.1016/0014-5793(85)80130-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The secondary transport systems of the yeast vacuolar membrane have been investigated by (i) the method of radioactive isotopes ([¹⁴C]arginine); (ii) activation of H⁺-ATPase by cations (Cat⁺), when the enzyme is under H⁺ control and (iii) measurement of changes in the proton gradient (Δ pH) and membrane potential (E _m) due to the supposed substrates of the transporters. The main mechanism of cation transport across the yeast tonoplast is probably H⁺/Cat⁺ antiport. The apparent K _m of antiporters for Ca²⁺, Mg²⁺ Mn²⁺, Zn²⁺ and P_i are 0.06, 0.3, 0.8, 0.055-0.17 and 1.5mM, respectively.

【 授权许可】

Unknown