Interpretation of fluorescence photobleaching recovery experiments on oriented cell membranes

FEBS Letters

Dale, R.E.¹ 

[1] Paterson Laboratories, Christie Hospital & Holt Radium Institute, Manchester M20 9BX, England

关键词: FPR;    FRAP;    Fluorescence polarization;    Lateral diffusion;    Rotational diffusion;    Mobile fraction;   

DOI  :  10.1016/0014-5793(85)80119-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Both quantitative and qualitative aspects of the interpretation of fluorescence photobleaching recovery experiments as typically practised to obtain information on lateral diffusion processes in cell membranes are called into question in view of the polarized nature of the laser light sources routinely employed. Protocols which will eliminate the effects elicited under these conditions by any concurrent slow rotational diffusion are delineated.

【 授权许可】

Unknown