Lipid domain in cancer cell plasma membrane shown by 1H NMR to be similar to a lipoprotein

FEBS Letters

Dyne, Marlen¹  Mountford, Carolyn E.¹  Helmer, Megan A.¹  Wright, Lesley C.¹  Fox, Richard M.¹  May, George L.¹  Williams, Philip G.¹  Holmes, Kerry T.¹ 

[1] Ludwig Institute for Cancer Research (Sydney Branch). Blackburn Building, University of Sydney, Sydney, NSW 2006, Australia

关键词: Lipoprotein;    t2D NMR;    Triglyceride;    (Cancer cell);    Plasma membrane;   

DOI  :  10.1016/0014-5793(85)80064-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human blood lipoproteins have been characterised by ¹H NMR methods and chemical analysis, and comparisons made with the properties of the triglyceride-rich plasma membrane domain found in cancer cells. By means of selective and non-selective T ₁ experiments, the lipids in HDL and LDL are shown to be in diffusive exchange. In contrast, the lipids ofchylomicra and VLDL do not exhibit lipid diffusion, and therefore resemble the neutral lipids of cancer cell plasma membranes. 2D scalar correlated NMR (COSY) spectra of cancer cells or solid tumours are similar to those obtained from VLDL and LDL. The long T ₂ relaxation value observed for neutral lipid methylenes in metastatic cancer cells (>00 ms) was not observed for any of the 4 lipoproteins studied. None of the lipoprotein classes gave a T ₂ longer than 250 ms.

【 授权许可】

Unknown