Further comments on the logic of the application of uncoupler‐inhibitor titrations for the elucidation of the mechanisms of energy coupling

FEBS Letters

Roy Caplan, S.¹  Pietrobon, Daniela¹ 

[1] Department of Membrane Research, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Uncoupler-inhibitor titration;    Chemiosmotic hypothesis;    Localized vs delocalized mechanism;    Proton pump Oxidative phosphorylation;    Energy coupling;   

DOI  :  10.1016/0014-5793(85)80055-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Following the appearance of two papers in this journal commenting on the logic of the application of uncoupler-inhibitor titrations as a means of discriminating between ‘delocalized’ and ‘localized’ chemiosmotic mechanisms [(1984) FEBS Lett. 176, 79-82; (1985) FEBS Lett. 186, 8-10], and in contrast with the arguments presented there and elsewhere, we show that in a linear model the increase in ΔgmH which accompanies partial inhibition of the ATPases always leads to a relatively higher decrease of the rate of ATP synthesis by a given concentration of uncoupler in the presence of an ATPase inhibitor than in its absence. This is due to the fact that the same titre of uncoupler leads to a higher dissipative H⁺ flow in the presence of inhibitor, since the driving force ΔgmH is higher.

【 授权许可】

Unknown