Oligomer distribution of depolymerase digests: comparison of theory and experiment

FEBS Letters

Nazarova, N.I.¹  Mazur, A.K.¹  Elyakova, L.A.¹ 

[1] Pacific Institute of Bioorganic Chemistry, Far East Science Centre, USSR Academy of Sciences, Vladivostok 690022, USSR

关键词: Enzyme degradation;    Product distribution;    Action pattern;    β-1;    3-Glucanase;    Transglycosylation;   

DOI  :  10.1016/0014-5793(85)80039-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Enzyme hydrolysis of linear β-1,3-glucan was carried out, with p-nitrophenylglucoside (in a high concentration) involved in simultaneous transglucosylation reaction. The p-nitrophenyloligosides were next separated by gel chromatography and the product distribution shape was studied. In good agreement with earlier theoretical predictions the distribution shape was close to an exponential one for oligomer chain length exceeding the size of the enzyme-binding region.

【 授权许可】

Unknown