Identification of proline carrier in Escherichia coli K‐12

FEBS Letters

Anraku, Yasuhiro¹  Yamato, Ichiro¹  Hanada, Kentaro¹ 

[1] Department of Biology, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan

关键词: putP gene;    Proline carrier;    N-[3H]Ethylmaleimide labeling;    Heat aggregation;    Ferguson plot;    Hydrophobic protein;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    MalNEt;    N-ethylmaleimide;    IPTG;    isopropyl-β-D-thiogalactopyranoside;   

DOI  :  10.1016/0014-5793(85)80024-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proline carrier, a product of the put P gene of Escherichia coli, was identified as a 35 kDa cytoplasmic membrane protein by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Its identification was based on the following evidence: First, the density of the band corresponding to a 35 kDa protein correlated with the proline-binding activity of cytoplasmic membranes from put P-deficient and put P-amplified strains. Second, by the differential labeling method, the 35 kDa protein was specifically labeled with radioactive N-ethyl-maleimide. The 35 kDa protein was found to aggregate on heat treatment and to show abnormal mobility on SDS-PAGE.

【 授权许可】

Unknown