Estimation of the rate of photochemical charge separation in Rhodopseudomonas sphaeroides reaction centers by fluorescence and absorption picosecond spectroscopy

FEBS Letters

Kononenko, Alexander A.¹  Korvatovskii, Boris N.¹  Rubin, Andrew B.¹  Paschenko, Vladimir Z.¹  Chamorovsky, Sergei K.¹ 

[1] Department of Biophysics, Faculty of Biology, M.V. Lomonosov State University, Moscow 119899, USSR

关键词: Rps. sphaeroides;    Reaction center;    Charge separation;    Picosecond spectroscopy;   

DOI  :  10.1016/0014-5793(85)80017-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Time-resolved fluorometry of reaction center (RC) preparations from Rhodopseudomonas sphaeroides, wild strain 1760-1, shows that the lifetime of the excited state of bacteriochlorophyll P870^∗ is τ = 6± 1.5 ps and independent of temperature within the range 293 – 77 K. This value was found to coincide well with the time (7 ± 3 ps) of the RC porphyrin pigment transition into the ion-radical pair state P^F, as measured by picosecond absorption spectroscopy of the same preparations.

【 授权许可】

Unknown