Quantitation of chordin in developing Huso huso embryos and larvae by radioimmunoassay

FEBS Letters

Glinka, A.V.¹  Preobrazhensky, A.A.¹ 

[1] A.N. Bakh Institute of Biochemistry, USSR Academy of Sciences, Leninsky pr. 33, 117071 Moscow, USSR

关键词: Chordin;    Tissue-specific protein;    Radioimmunoassay;    Type II collagen;    Embryonic differentiation marker;   

DOI  :  10.1016/0014-5793(85)80998-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chordin is a protein discovered in the notochord cells of the representatives of Acipenseridae; giant sturgeon, stellate sturgeon and sterlet. Some characteristics of the purified chordin preparation which justify its use in radioimmunoassay are described. A sensitive competitive-binding double-antibody radioimmunoassay for chordin is described by which its content in the extracts from giant sturgeon embryos and larvae has been measured. It is shown that chordin biosynthesis started in the embryos from stage 32.

【 授权许可】

Unknown